Journal: bioRxiv

Article Title: FoxO factors are essential for maintaining organ homeostasis by acting as stress sensors in airway epithelial cells

doi: 10.1101/2024.01.31.578231

Figure Lengend Snippet: A) Relative levels of hFOXO factors in sputum samples of asthmatic patients and non-asthmatic controls were quantified by mRNA microarray analyses (n≥3). Statistical analysis was performed by the Mann-Whitney U test. B) NHBEs of healthy individuals were cultured under control conditions or treated with IL-4 to induce Th1/Th2 priming. Relative hFOXO transcripts were quantified by mRNA microarray analyses (n=6). Statistical analysis was performed by the Mann-Whitney U test. *p<0.05 and **p<0.01. C) Scheme summarizing the function of FoxO factors in a physiological state (left) or in a disease-associated non-physiological state (right).

Article Snippet: Samples were quantified with a fluorometric dye (Quant-IT, Thermofisher, USA) and between 187 and 500 ng per sample were used as input for the TruSeq stranded mRNA library kit (Illumina, USA) following the manufacturers manual.

Techniques: Microarray, MANN-WHITNEY, Cell Culture, Control